Enzyme-linked Immunosorbent Assay (ELISA)

Individual protein levels from biological samples may be quantified by employing enzyme-linked immunosorbant assay (ELISA). Samples may include serum, plasma, saliva, culture supernatants or cell lysates.  Sample protein is sandwiched between capture and detection antibodies against a protein of interest.  Horseradish peroxidase (HRP) linked to either the detection antibody or a secondary antibody chemically reacts with a chromogen, prompting a color change that can be measured by spectrophotometry.  Fluorescent output from samples is compared to that from recombinant protein standards, to provide a final sample concentration.  AcuImmune scientists have experience working with the following kits and assay formats:

  • Preclinical Drug Development
    • Human Therapeutic IgG2: Measure humanized IgG antibody therapeutics circulating in human subjects for pharmacokinetic (PK) studies.  This assay is capable of differentiating between mAb therapeutics and naturally produced antibodies.
    • Antidrug Antibodies (ADA): Quantify host-produced antibodies against mAb therapeutics for immunogenicity studies.  Our method can characterize drug-neutralizing antibodies as against CDR or Constant-Framework portions of antibodies.
  • Protein Measurement
    • Immune Cytokines: IFNɣ, IL-2, IL-10, and more from animal and human serum, saliva, and cell-culture supernatant.
    • Biomarker Quantification: Measure intracellular (cell lysates) and circulating proteins (serum) to characterize disease pathogenesis.